Journal: Scientific Reports

Article Title: Gender Differences in Bile Acids and Microbiota in Relationship with Gender Dissimilarity in Steatosis Induced by Diet and FXR Inactivation

doi: 10.1038/s41598-017-01576-9

Figure Lengend Snippet: Histological and phenotypic changes in control diet and Western diet-fed wild type and FXR KO mice of both genders. ( A ) Body weight gain. ( B ) Percentage of liver to body weight ratio. ( C ) Serum alanine aminotransferase (ALT). ( D ) Serum endotoxin level. ( E ) Representative liver morphology and H&E-stained liver sections. Scale bars, 100 µm. Hepatic triglycerides ( F ) and cholesterol ( G ) level. Serum triglycerides ( H ) and cholesterol ( I ) level. n = 6 per group. Data are expressed as mean ± SD. One-way ANOVA with Tukey’s correction. * p < 0.05, ** p < 0.01, *** p < 0.001 for diet comparison; # p < 0.05, ## p < 0.01, ### p < 0.001 for genotype comparison; & p < 0.05, && p < 0.01, &&& p < 0.001 for gender comparison.

Article Snippet: Serum alanine aminotransferase (ALT, Pointe Scientific), lipopolysaccharide (LPS, Thermo Scientific), serum and hepatic triglyceride and cholesterol (BioAssay Systems) levels were quantified according to the manufacturer’s instructions.

Techniques: Control, Western Blot, Staining, Comparison

Journal: The Journal of nutritional biochemistry

Article Title: Synbiotics Bifidobacterium infantis and milk oligosaccharides are effective in reversing cancer-prone non-alcoholic steatohepatitis using Western diet-fed FXR knockout mouse models

doi: 10.1016/j.jnutbio.2018.04.007

Figure Lengend Snippet: (A) Hematoxylin and eosin (H&E) staining of liver, (B) hepatic lymphocyte score, (C) serum ALT level, (D) steatosis score, (E) hepatic triglyceride, and (F) hepatic cholesterol levels of diet and gender-matched mice supplemented with and without B. infantis, MO, and B. infantis plus MO for 7 months. Data expressed as mean±SD. n ≥ 6 per group. *p<0.05, **p<0.01, WT mice compared with FXR KO mice, and untreated FXR KO mice compared with treated FXR KO mice.

Article Snippet: Biochemical assays and histology Serum alanine aminotransferase (ALT; Pointe Scientific, Canton, MI, USA), serum alkaline phosphatase (ALP; Pointe Scientific), hepatic triglyceride, and hepatic cholesterol (BioAssay Systems, Hayward, CA, USA) levels were quantified according to the manufacturer’s instructions.

Techniques: Staining

Journal: Poultry Science

Article Title: Research note: Effects of dietary bile acids supplementation on hepatic lipid accumulation and fatty acid profile of hens at late laying cycle

doi: 10.1016/j.psj.2025.106026

Figure Lengend Snippet: Effects of bile acids supplementation on liver histopathology and mRNA expression of lipid metabolism-related genes in the hens at late laying cycle. (A) Hematoxylin and eosin photomicrographs obtained at 40 × (Ⅰ-Ⅳ) and 400 × (Ⅴ-Ⅷ) magnification; (B) HSL , hormone-sensitive triglyceride lipase; (C) SCD , stearoyl-CoA desaturase-1; (D) ADD1 , adipocyte determination and differentiation factor-1; (E) LPL , lipoprteinlipase; (F) FAS , fatty acid synthetase; (G) FABP4 , fatty acid-binding protein 4. Control, BA200, BA300, and BA500 represent the basal diet added with 0, 200, 300, and 500 mg/kg bile acids, respectively. a,b Means with different lowercase letters differ significantly among the four treatments ( P < 0.05). Lin and Quad showed linear and quadratic effects of different dietary BAs levels. n = 6.

Article Snippet: Hepatic triglyceride ( TC ; CAS#A110-1-1), total cholesterol ( TG ; CAS#A111-1-1), and MDA (CAS#A003-1-2) levels were measured using the commercial assay kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer's protocols.

Techniques: Histopathology, Expressing, Binding Assay, Control